My 260/280 ratios seem ok (> 1.7) , however my 260/230 ratio is below 1. Is 260/230 ratio absolutely necessary or just 260/280 ratios are enough for NGS. I have tried PCI, ethanol precipitation and also used sillica column based kits but with little success.
http://www.nanodrop.com/Library/T009-NanoDrop%201000-&-NanoDrop%208000-Nucleic-Acid-Purity-Ratios.pdf
A low A 260/A230 ratio could be by high concentrations of carbohydrate (if you used glycogen in the extraction or by their presence in samples), or by traces of phenol or guanidine (possibly by the type of extraction method).
On the other hand, from my experience, if you have a lower quality of 1.2 it is likely to fail . Anyway try performing a PCR with primers that you've chosen. After all, if amplified is likely to it no give more problems.
Even so, at this link you can find more information:
https://www.researchgate.net/post/What_is_the_effect_of_a_low_260_230_ratio_on_the_purity_of_DNA
Best Regards
Thank you for your valuable suggestions..Any other thoughts on how should I purify my DNA to get 260/A230 ratio in an acceptable range.
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