Asim Dutta

3 Questions 8 Answers 0 Followers

Questions related from Asim Dutta

Web MD Simulation?

Is there any other online MD web simulation sites similar to MDWeb server.

11 September 2018 7,846 3 View

What is the ideal 260/280 and 260/230 ratios for DNA for next generation sequencing?

My 260/280 ratios seem ok (> 1.7) , however my 260/230 ratio is below 1. Is 260/230 ratio absolutely necessary or just 260/280 ratios are enough for NGS. I have tried PCI, ethanol precipitation...

30 June 2016 4,294 3 View

What is the ideal percentage of total protein content in an overexpressed E.coli cell?

I am overexpressing a protein BL21 ecoli cell. The total protein content after cell lysis is about 50% of the wet weight (crude cell pellet). Isn't this bit high??

19 May 2015 4,406 11 View