I am looking to know the IC50 of podophyllotoxin in MCF-7, HeLa or other cell lines, in literature I found as positive control etoposide or teniposide but never the podophyllotoxin.
I've worked with MCF7 cells developing some cytotoxic assays. For my experiments, I couldn't find the IC50 for the drugs that I used. So, my advice is to set up a pilot experiment, using different cell concentrations exposed at different podophyllotoxin's concentrations during 1, 3, 5, 10, 18, 24, 48 and 72 h. This can be done in a 96-well plate. You can also find a good resource how to set up these experiments in the book: "Culture of animal cells" by Ian Freshney. Hope this helps!
You can check the following reference although not specific to MCF7 cells: H. Chen, S. Zuo, X. Wang, X. Tang, M. Zhao, Y. Lu, L. Chen, J. Liu, Y. Liu, D. Liu, S. Zhang, T. Li, Synthesis of 4’-triazole-podophyllotoxin derivatives by azide-alkyne cycloaddition and biological evaluation as potential antitumor agents, Eur. J. Med. Chem. 46 (2011) 4709–4714. doi:10.1016/j.ejmech.2011.07.024.
- Badges
- Science topic