How temperature affect plasmid DNA conformation?

I conduct a thermal stability test for plasmid DNA. In which I put plasmid DNA under 65-degree celsius for about one week. After that, I run an agarose gel check. Here below is what I got: 3 left...

11 December 2016 8,159 4 View

What is the reason for the complete loss of transformation efficiency?

I have conducted a test on transformation efficiency of circular plasmid DNA after some harsh storage period (in which I put the plasmid DNA in 60 Celcius degree for 2 weeks). And results showed...

09 October 2016 3,275 4 View

Linearized and Circular Plasmid DNA, Which is more stable under same storage condition?

I would like to ask if someone has any experience or Ref on the stability comparison of the above said plasmid DNA (linearized and circular plasmid DNA, stored at same storage conditions). What...

09 October 2016 9,270 6 View

What are the methods for nucleotide ratio quantitation?

Providing that I have a single stranded DNA (short fragment ~30 nt) with unknown sequence. What are the experimental methods which could help to determine nucleotide ratio (percentage of A,T,G,C)...

03 April 2016 314 2 View

How to measure adsorption of biomolecules in liquid on solid surface?

03 April 2016 2,400 3 View

What is the problem with my bacterial cell growth in LB media?

02 March 2016 3,904 7 View

What universal primer should I choose for plasmid sequencing?

11 December 2015 2,223 6 View

Can TA TOPO Cloning vector be replaced for specific vector in cloning?

I would like to ask if TA TOPO cloning vector can be used as alternative for plasmid vector such as pet21, pet28 .etc. And in that case, why should we bother using specific plasmid vectors...

10 November 2015 8,046 2 View

What are the differences between Nanoparticles and Nanopowder?

I am in need of using Manganese nanoparticles but not quite familiar with the synthesis. There is nanopowder of manganese oxide commercially available. In this case, could I disperse nanopowder...

10 November 2015 3,996 5 View

Could an incorrect dilution affect the reaction outcome?

I think there is a difference between these 2 dillution methods and therefore it might affects the reaction (A+B=AB). Could you please give me some clear explainations on this: I have: Stock A: 10...

31 December 2014 2,909 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View