H.H Nguyen

12 Questions 25 Answers 0 Followers

Questions related from H.H Nguyen

How temperature affect plasmid DNA conformation?

I conduct a thermal stability test for plasmid DNA. In which I put plasmid DNA under 65-degree celsius for about one week. After that, I run an agarose gel check. Here below is what I got: 3 left...

12 December 2016 8,215 4 View

Linearized and Circular Plasmid DNA, Which is more stable under same storage condition?

I would like to ask if someone has any experience or Ref on the stability comparison of the above said plasmid DNA (linearized and circular plasmid DNA, stored at same storage conditions). What...

10 October 2016 9,349 6 View

What is the reason for the complete loss of transformation efficiency?

I have conducted a test on transformation efficiency of circular plasmid DNA after some harsh storage period (in which I put the plasmid DNA in 60 Celcius degree for 2 weeks). And results showed...

10 October 2016 3,327 4 View

What are the methods for nucleotide ratio quantitation?

Providing that I have a single stranded DNA (short fragment ~30 nt) with unknown sequence. What are the experimental methods which could help to determine nucleotide ratio (percentage of A,T,G,C)...

04 April 2016 398 2 View

How to measure adsorption of biomolecules in liquid on solid surface?

I would like to ask what techniques I could use to measure / monitor the adsorption of DNA on solid surfaces (In my case, it is metal nanoparticles)?

04 April 2016 2,488 3 View

What is the problem with my bacterial cell growth in LB media?

I am facing a weird situation where I got my ligated plasmid in DH5α growing well on the streak plate (overnight), but after I transfered the cells from the streak plate to LB medium, they grow...

03 March 2016 3,957 7 View

What universal primer should I choose for plasmid sequencing?

I ligated my insert into the between of Xho I and Nde I of pet28a vector (see image), and I am wonderfing if T7 promoter or T7 terminator should be chosen as the primer for sequencing. Could you...

12 December 2015 2,275 6 View

Can TA TOPO Cloning vector be replaced for specific vector in cloning?

I would like to ask if TA TOPO cloning vector can be used as alternative for plasmid vector such as pet21, pet28 .etc. And in that case, why should we bother using specific plasmid vectors...

11 November 2015 8,097 2 View

What are the differences between Nanoparticles and Nanopowder?

I am in need of using Manganese nanoparticles but not quite familiar with the synthesis. There is nanopowder of manganese oxide commercially available. In this case, could I disperse nanopowder...

11 November 2015 4,045 5 View

Could an incorrect dilution affect the reaction outcome?

I think there is a difference between these 2 dillution methods and therefore it might affects the reaction (A+B=AB). Could you please give me some clear explainations on this: I have: Stock A: 10...

01 January 2015 2,970 3 View

EcoR I does not fully digest my DNA. Why?

I used a 80 bp ds DNA which contains EcoR I recognition site in the middle of the strain, my objective is to obtain 40 bp ds DNA fragment. I used the protocol supplied with my enzyme (from...

08 August 2014 7,394 8 View

What is the form of synthesized oligonucleotides, A or B?

Native DNA in cells is known to be in B form, mostly. What about synthesized one?

06 June 2014 5,165 3 View