How temperature affect plasmid DNA conformation?

I conduct a thermal stability test for plasmid DNA. In which I put plasmid DNA under 65-degree celsius for about one week. After that, I run an agarose gel check. Here below is what I got: 3 left...

11 December 2016 8,159 4 View

What is the reason for the complete loss of transformation efficiency?

I have conducted a test on transformation efficiency of circular plasmid DNA after some harsh storage period (in which I put the plasmid DNA in 60 Celcius degree for 2 weeks). And results showed...

09 October 2016 3,275 4 View

Linearized and Circular Plasmid DNA, Which is more stable under same storage condition?

I would like to ask if someone has any experience or Ref on the stability comparison of the above said plasmid DNA (linearized and circular plasmid DNA, stored at same storage conditions). What...

09 October 2016 9,270 6 View

How to measure adsorption of biomolecules in liquid on solid surface?

03 April 2016 2,400 3 View

What is the problem with my bacterial cell growth in LB media?

02 March 2016 3,904 7 View

What universal primer should I choose for plasmid sequencing?

11 December 2015 2,223 6 View

Can TA TOPO Cloning vector be replaced for specific vector in cloning?

I would like to ask if TA TOPO cloning vector can be used as alternative for plasmid vector such as pet21, pet28 .etc. And in that case, why should we bother using specific plasmid vectors...

10 November 2015 8,046 2 View

What are the differences between Nanoparticles and Nanopowder?

I am in need of using Manganese nanoparticles but not quite familiar with the synthesis. There is nanopowder of manganese oxide commercially available. In this case, could I disperse nanopowder...

10 November 2015 3,996 5 View

Could an incorrect dilution affect the reaction outcome?

I think there is a difference between these 2 dillution methods and therefore it might affects the reaction (A+B=AB). Could you please give me some clear explainations on this: I have: Stock A: 10...

31 December 2014 2,909 3 View

EcoR I does not fully digest my DNA. Why?

I used a 80 bp ds DNA which contains EcoR I recognition site in the middle of the strain, my objective is to obtain 40 bp ds DNA fragment. I used the protocol supplied with my enzyme (from...

07 August 2014 7,337 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View