How temperature affect plasmid DNA conformation?

I conduct a thermal stability test for plasmid DNA. In which I put plasmid DNA under 65-degree celsius for about one week. After that, I run an agarose gel check. Here below is what I got: 3 left...

11 December 2016 8,159 4 View

What is the reason for the complete loss of transformation efficiency?

I have conducted a test on transformation efficiency of circular plasmid DNA after some harsh storage period (in which I put the plasmid DNA in 60 Celcius degree for 2 weeks). And results showed...

09 October 2016 3,275 4 View

Linearized and Circular Plasmid DNA, Which is more stable under same storage condition?

I would like to ask if someone has any experience or Ref on the stability comparison of the above said plasmid DNA (linearized and circular plasmid DNA, stored at same storage conditions). What...

09 October 2016 9,270 6 View

What are the methods for nucleotide ratio quantitation?

Providing that I have a single stranded DNA (short fragment ~30 nt) with unknown sequence. What are the experimental methods which could help to determine nucleotide ratio (percentage of A,T,G,C)...

03 April 2016 314 2 View

What is the problem with my bacterial cell growth in LB media?

02 March 2016 3,904 7 View

What universal primer should I choose for plasmid sequencing?

11 December 2015 2,223 6 View

Can TA TOPO Cloning vector be replaced for specific vector in cloning?

I would like to ask if TA TOPO cloning vector can be used as alternative for plasmid vector such as pet21, pet28 .etc. And in that case, why should we bother using specific plasmid vectors...

10 November 2015 8,046 2 View

What are the differences between Nanoparticles and Nanopowder?

I am in need of using Manganese nanoparticles but not quite familiar with the synthesis. There is nanopowder of manganese oxide commercially available. In this case, could I disperse nanopowder...

10 November 2015 3,996 5 View

Could an incorrect dilution affect the reaction outcome?

I think there is a difference between these 2 dillution methods and therefore it might affects the reaction (A+B=AB). Could you please give me some clear explainations on this: I have: Stock A: 10...

31 December 2014 2,909 3 View

EcoR I does not fully digest my DNA. Why?

I used a 80 bp ds DNA which contains EcoR I recognition site in the middle of the strain, my objective is to obtain 40 bp ds DNA fragment. I used the protocol supplied with my enzyme (from...

07 August 2014 7,337 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View