Could someone help me that how Ag NPs act as antimicrobial? Weather it bind with cell wall, DNA or protein…..as up till now we are just supposing that it may inhibit cell wall, may be bind with sulphur or phosphorus of DNA and protein or as free radical.
Dear Muhammad,
You can find a lot of information about how AgNPs works in this recent pubblication.
Best regards.
Article ChemInform Abstract: Nanosilver-Based Antibacterial Drugs an...
ROS attack on the cell membrane as well on other biomolecules of microbes
Nanoparticles produce reactive oxygen species. Which are antimicrobial.
Free redical itself a toxic. Then how we will use its as antimicrobial Sami Khan
Silver nanoparticles have the ability to anchor to the bacterial cell wall and subsequently penetrate it, thereby causing structural changes in the cell membrane like the permeability of the cell membrane and death of the cell. There is formation of ‘pits’ on the cell surface, and there is accumulation of the nanoparticles on the cell surface
The formation of free radicals by the silver nanoparticles may be considered to be another mechanism by which the cells die. There have been electron spin resonance spectroscopy studies that suggested that there is formation of free radicals by the silver nanoparticles when in contact with the bacteria, and these free radicals have the ability to damage the cell membrane and make it porous which can ultimately lead to cell death
It has also been proposed that there can be release of silver ions by the nanoparticles, and these ions can interact with the thiol groups of many vital enzymes and inactivate them. The bacterial cells in contact with silver take in silver ions, which inhibit several functions in the cell and damage the cells. Then, there is the generation of reactive oxygen species, which are produced possibly through the inhibition of a respiratory enzyme by silver ions and attack the cell itself. Silver is a soft acid, and there is a natural tendency of an acid to react with a base, in this case, a soft acid to react with a soft base
The cells are majorly made up of sulfur and phosphorus which are soft bases. The action of these nanoparticles on the cell can cause the reaction to take place and subsequently lead to cell death. Another fact is that the DNA has sulfur and phosphorus as its major components; the nanoparticles can act on these soft bases and destroy the DNA which would definitely lead to cell death
Ag have strong attraction towards sulfur and phosphorous present in cell membrane and form mercaptan.
Ofcourse but I means how it act as antibacterial. Cidal or static ?
Silver have strong affinity towards Sulphur & Phosphorus present in the cell wall
Silver nanoparticles produce Reactive Oxygen Species and free radicals
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