In XRD some other peaks appeared mostly

You can use XRD for determination of nanoparticles shape

However TEM or HRTEM will be best

In XRD some other peaks appeared mostly

Then that will show some impurities in your samples

Of-course

XRD, SEM, AFM and TEM can provide information about the shape and size of the nanoparticles.

TEM & AFM are not available with us

SEM also can give information about size in two dimensional.

Thanks Virendra Nath Rai

HR-TEM, TEM or FESEM

XRD also provide the average crystalline size of nano using Sherrer Equ

I have already done XRD

Thanks for your response Fazal Hussain

From XRD using Sherrer Equ

Yeah Thanks but thats average size

Yes XRD provide you average size. For the exact size you can use AFM or TEM

Ok

You can confirm it from XRD also

XRD gave exact size or average size ?

XRD give average size using Sherrer Equation. TEM or HRTEM will be best

TEM is batter but if XRD is available then that also work

In XRD mostly some unidentified peaks were observed in plant mediated silver nanoparticles

Compare your XRD pattern with literature. If not found that peaks then that will due to extract

Compared as I have mentioned earlier. One peak of that is due to oxide and the next one is unidentified

The next one will be due to crystallization of biomass

Sami Khan how you can say that the other peak will be due to biomass ?

Thanks Sami Khan & Zahid Ali Khan

Dear Zahid

As reported in the literature

These unassigned peaks in AgNPs pattern (18.3º, 20.35º, 24.75º) are due to the crystallization of bio-organic molecules that act as capping agents which are present in the extract [Colloids and Surfaces B: Biointerfaces, 76 (2010) 50-56].

So We can say that the unrecognized peaks may be due to the crystallization of biomass

Yes sure. Thanks for suggestions

Thanks agree

You can used XRD if you have no access to TEM

Thanks but that is average size

FE-SEM can also be used for the size

Something is better then nothing

You are right

As mentioned Sami Khan you can also use FE-SEM for the size

Yes many researcher use FE-SEM for the size determination of nanoparticles

Use XRD

But XRD size is average size

You can also use FE-SEM for size

FE-SEM also give exact size

FESEM, TEM and XRD all could be used. Best is TEM or HRTEM

TEM or HRTEM will be best

Thanks Thahir. But in Pakistan I search too much....non of university have TEM

For exact size you will use TEM

TEM is available at Peshawar Univ

That is out of order dear

You can use TEM and also XRD

I have no access to TEM

You can also use FE-SEM

I have used that

Yar what about the TEM of Faisalabad ?

You can use SEM, with XRD and DLS

SEM, AFM. An equation is used too when XRD is applied

Omar Farhan Ammar thanks

Abdounasser Omar that is Sherrer equation but that is average size

Agree with @Omar Farhan Ammer

If you have access to dynamic light scattering DLS then that might be a nice method. There is also Nanoparticle tracking analysis NTA that can provide size information: https://www.materials-talks.com/blog/2016/09/15/nta-or-dls/

Thanks Dr Ulf Nobbmann

HR TEM

TEM or HRTEM

HRTEM is best

XRD ??

Thanks Zahid Ali Khan

Thanks Tahir Saeed Khan

Thanks Sami Khan

Have you used XRD ?

Yes used it but getting rough size.

Yes

How rough ?

HRTEM

Not rough. Average

Yes average but not sure

Dr TEM is available in CRL also

That is not working.

Out of order

Faisalabad me bi hai

Thats is also not responding

Space technology lab Islamabad have TEM facility

That is good. I will ask

Yes Dr I also known from some one about that

Have you ask in GIK Sawabi ?

No access to GIK

Fazal Hussain

I think XRD will be enough

Have done

Use FESEM

Yes FESEM could be used if give you clear images

Follow

Thanks Zahid Ali Khan

Thanks Tahir Saeed Khan

Thanks Sami Khan

Hello Muhammad Ismail ,

If You need to know the size distribution of your nanoparticles, but image is not mandatory, You can use light scattering techniques. Companies like Malvern, Wyatt or Brookhaven Instruments developed some machines for size and zetapotential measurements, which can determine also size distributions. Some of them are not very precise, but cheap and commonly used in the number of laboratories. The most common ones - Zetasizer and Mastersizer series form Malvern.

Static and dynamic light scattering (SLS and DLS) are the most sensitive and precise techniques for the particle size measurements, but there are some issues like special sample preparation routes. But still it depends on the nature of Your sample.

I hope this answer will help You.

Thanks Ivan Shepa

Dynamic light scattering technique or XRD would be good enough to estimate the particle

Thanks Abhishek Babu

Recommend Abhishek Babu

Dynamic light scattering technique

Thanks