Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

Silane solution and application to Glass ?

30 January 2021 7,924 3 View

Do you think that GIS is going to vanish in next ten years or visa versa?

Smart application and Google earth and google maps are now becoming a huge geo spatial data base and this may decrease the use of software related to GIS

29 December 2020 3,940 12 View

I how to produce w1/o/w2 using silicone oil as o phase and that with different ratio of inner water to oil as well as different interfacial areas?

hi everyone, the challenge in my project is to produce double emulsion with exactly the same material system but with different ratios of inner water to oil (silicone oil as o phase)as well as...

22 July 2020 5,223 3 View

What is a good international journal to publish a paper in applied linguistics?

Based on personal experience, I need suggestions for an international journal to publish my paper. I need a journal that is responsive, has a good impact factor and has a print version. Thank you.

02 March 2020 5,400 4 View

Calcule the refractive index and extinction coefficient of multilayers?

hi, how can I calculate the equivalent refractive index and equivalent extinction coefficient of multilayers from the optical information of each layer, is there any simple software to calculate...

06 November 2019 5,507 6 View

How to define PEDOT: PSS in Silvaco?

I found a problem to define an organic material which is PEDOT: PSS in silvaco , could anyone helps me out?

26 June 2019 7,483 1 View

What are the merits of ACP (Ansys composite prepost) other than the ansys apdl?

need the merits of ACP

15 June 2019 4,613 3 View

Switched overlapping estimators technique and How we can run two estimators simultaneously but in an overlapping fashion?

Dear all, Please, i need a helpful example to examine the issue of how and when to update, or re-initialize, the computations with SIMULINK or Matlab. How we can run two estimators...

18 October 2018 6,868 3 View

Can anyone suggest a technique to temporarily store brain tissue if my end goal is to grow primary microglia cultures?

Hi, I am interested in examining the effects of an endotoxin on microglial cells. I will be harvesting brains from mice at p2, treating them with this factor and then performing some molecular...

27 September 2018 1,793 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

What is the most efficient mouse brain weight for RNA extraction for NGS?

I have collected the brains and they are Fresh Frozen into PFC, Mid Brain and Brain Stem. I want to extract the amygdala and hypothalamus.

01 January 2021 7,833 1 View

Help regarding free-floating immunohistochemistry on mouse spleen?

Hello fellow researchers I am trying to stain cryostat-sectioned spleen tissue, using free-floating chromogenic immunohistochemistry. Unfortunately, I'm not able to detect any staining (MHC class...

13 November 2020 6,884 3 View

What is the value of dynamic viscosity and bulk viscosity of extracellular matrix (ECM), nucleus, and cytoplasm of cell?

Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of...

19 October 2020 8,729 1 View

How transfected DNA is transported into the nucleus, to get stable integration?

What kind of mechanisms are involved in the integration and transportation of transfected DNA/Gene to the nucleus?

08 October 2020 2,329 2 View

What is the best way to detect ATF4 activation? And ATF6?

To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you

17 September 2020 2,973 1 View

Why COS 7 Cells change its morphology to round shape (rounded nucleus and peri nucleus cytoplasm) after Transfection?

I have transfected COS 7cells to express Protein-GFP conjugate, transfection rate was very low (20-30 %) , but the cell morphology changed, many of the cells appear round, (nucleus round too)...

15 September 2020 5,324 3 View

Can bacteria and biofilm on a filter be seen with SEM without a dehydration/fixation step?

I have a filter with some bacteria and other particles that is going to be analyzed with SEM. I am wondering if anyone knows if you skip the glutaraldehyde/ethanol/critical point drying steps if...

01 September 2020 4,929 4 View