Is there any analyzing software or sizing software for mechanical seal?

I wonder if there would be a software Specialized for analyzing or sizing mechanical seals excluding ANSYS , Fluent or COMSOL.

07 August 2017 5,724 1 View

A Problem in PCR?

Hi there, In my experiment I need to do PCR with below DNA and Primers, I also use RT-PCR.But I don't know why it cant be amplified. The other information is available in the attached...

09 October 2016 9,021 14 View

Is there an effective way to confirm ligation ?

Hi there,In my experiment I need to ligate 3 ssDNA, 80bp (S123) with help of 2 other ssDNA 80 bp as bridge (NS12).this assembly uses single-stranded bridging oligos complementary to the ends of...

09 October 2016 4,533 10 View

Is there any way to confirm ligation and hybridization?

07 August 2016 7,205 2 View

Is there anyway to remove DTT from recombination protein preparation with high efficiency and within an hour?

07 August 2016 1,736 4 View

Is PAGE required for normal PCR?

Hi there~ In my experiment below steps are required and five fragments has been designated, to order this oligos I select the normal desalting purification but the company recommended me to add...

05 June 2016 950 2 View

What is the low cost and easy way to label protein (T4 DNA Ligase) with RhodamineB?

In my research it is required to label T4 DNA Ligase with Rhodamine B, is there anyone who recommend a low cost protocol for labeling.

01 February 2016 5,632 0 View

No title

01 February 2016 8,989 3 View

Why does the center of polymer not cure well during polymerization?

I would like to apprecieate your comments, i updated this post with more details In this work i have to do local polymerization in a micro channel the heigth of micro channel is 12um and the size...

31 December 2015 3,306 5 View

Is there any possibility to use Real time PCR as normal End point PCR?

31 December 2015 7,553 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View