Ali Karimi

13 Questions 15 Answers 0 Followers

Questions related from Ali Karimi

Is there any analyzing software or sizing software for mechanical seal?

I wonder if there would be a software Specialized for analyzing or sizing mechanical seals excluding ANSYS , Fluent or COMSOL.

08 August 2017 5,768 1 View

A Problem in PCR?

Hi there, In my experiment I need to do PCR with below DNA and Primers, I also use RT-PCR.But I don't know why it cant be amplified. The other information is available in the attached...

10 October 2016 9,064 14 View

Is there an effective way to confirm ligation ?

Hi there,In my experiment I need to ligate 3 ssDNA, 80bp (S123) with help of 2 other ssDNA 80 bp as bridge (NS12).this assembly uses single-stranded bridging oligos complementary to the ends of...

10 October 2016 4,574 10 View

Is there anyway to remove DTT from recombination protein preparation with high efficiency and within an hour?

In my experiment DTT removal with high efficiency and in short time is required therefore doing dialysis may not be suitable for my work. does any person know a to remove DTT with high efficiency...

08 August 2016 1,778 4 View

Is there any way to confirm ligation and hybridization?

Hi there,There are five DNA fragments(5’ phosphorylated) in which some sequences between them are same and after mixing together they may assembled together as they have similar sequences, it...

08 August 2016 7,246 2 View

Is PAGE required for normal PCR?

Hi there~ In my experiment below steps are required and five fragments has been designated, to order this oligos I select the normal desalting purification but the company recommended me to add...

06 June 2016 998 2 View

What is the electrophoretic mobility of ATP and DTT?

02 February 2016 6,820 2 View

Is there anyway to block the 3'-end of an oligonucleotide after ligation (rather than heating at 65°C)?

In my experiment after ligation process It is required to block the 3' end of oligonucleotide from polymerase extension.Normally after the ligation heating will deactivate the ligation process...

02 February 2016 9,056 3 View

What is the low cost and easy way to label protein (T4 DNA Ligase) with RhodamineB?

In my research it is required to label T4 DNA Ligase with Rhodamine B, is there anyone who recommend a low cost protocol for labeling.

02 February 2016 5,711 0 View

Is there any possibility to use Real time PCR as normal End point PCR?

In my experiment using PCR and Gel Electrophoresis is required, since in our school only Real time PCR is available , I wounder if there would be a possibility to use Real time PCR to do my...

01 January 2016 7,603 4 View

Why does the center of polymer not cure well during polymerization?

I would like to apprecieate your comments, i updated this post with more details In this work i have to do local polymerization in a micro channel the heigth of micro channel is 12um and the size...

01 January 2016 3,350 5 View

Is there any water soluble highly sensitive photo-initiator?

In my research having a highly sensitive photo-initiator is required in the range of 300~400 nm is there any water soluble (at least 2%) photo-initiator with this specification?

12 December 2015 3,933 4 View

How to use conjugate heat transfer in CFX and fluent?

I am solving the conjugate heat transfer problem in a steady flow meter. The definition of the problem is: Suppose there is pressure. In this configuration, the heat transfer steam is flowing in...

11 November 2013 4,623 1 View