A Problem in PCR?

Hi there, In my experiment I need to do PCR with below DNA and Primers, I also use RT-PCR.But I don't know why it cant be amplified. The other information is available in the attached...

09 October 2016 9,021 14 View

Is there an effective way to confirm ligation ?

Hi there,In my experiment I need to ligate 3 ssDNA, 80bp (S123) with help of 2 other ssDNA 80 bp as bridge (NS12).this assembly uses single-stranded bridging oligos complementary to the ends of...

09 October 2016 4,533 10 View

Is there any way to confirm ligation and hybridization?

07 August 2016 7,205 2 View

Is there anyway to remove DTT from recombination protein preparation with high efficiency and within an hour?

07 August 2016 1,736 4 View

Is PAGE required for normal PCR?

Hi there~ In my experiment below steps are required and five fragments has been designated, to order this oligos I select the normal desalting purification but the company recommended me to add...

05 June 2016 950 2 View

What is the low cost and easy way to label protein (T4 DNA Ligase) with RhodamineB?

In my research it is required to label T4 DNA Ligase with Rhodamine B, is there anyone who recommend a low cost protocol for labeling.

01 February 2016 5,632 0 View

What is the electrophoretic mobility of ATP and DTT?

What is the electrophoretic mobility of ATP and DTT?

01 February 2016 6,745 2 View

No title

01 February 2016 8,989 3 View

Why does the center of polymer not cure well during polymerization?

I would like to apprecieate your comments, i updated this post with more details In this work i have to do local polymerization in a micro channel the heigth of micro channel is 12um and the size...

31 December 2015 3,306 5 View

Is there any possibility to use Real time PCR as normal End point PCR?

31 December 2015 7,553 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View