What is the doubling time of T84 cell line? What should be the seeding density of T84 in a 96 well plate?

07 June 2023 1 5K Report

I seeded 8000 cells per well in 1 96 well plate. The number I think is very high for T84. So, I need the doubling time of T84.

Amos Bairoch

I was lacking in the Cellosaurus entry the DT for T84, however I uncovered a paper:

https://pubmed.ncbi.nlm.nih.gov/25944804/

Which has DT for T84 of 33.9 hours in the supplemental table 1. This informaation and the other 52 cell line doubling times reported by this publication will be added to the next release of the Cellosaurus. So your question was very useful, thank you!

Best regards

Amos

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