Can I use BL21(DE3) strain for library preparation ?

Matruprasad Mohanty @Matruprasad-Mohanty

28 May 2024 0 5K Report

I am creating enzyme variant library through random mutagenesis but I am not getting any colonies after transformation using ligated product. I have E. coli BL21 (DE3) and BL21 Rosetta 2 (DE3) cells with me. I have tried heat shock method and electroporation but no colonies appeared. Should I try any other strains or change the transformation method?

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