Why does my RT-qPCR reaction of microRNA shows amplification only after 40 cycles?

I am trying to construct a standard plot for miR21 and miR 200 (Cq vs conc.) using synthetic oligos but even on using miRNA concentrations of upto 100pM I only begin to get any amplification...

08 September 2019 5,927 9 View

Does the particle size reported with AFM images actually mean their height? Or is it the diameter?

I have tried depositing metal colloidal films on glass slides (paper attached). They reported particles sizes using AFM image analysis which is very small compared to what I have got. It could be...

01 February 2019 1,951 11 View

I need to experimentally determine the psf of my microscope using fluorescent beads?

I have captured the z-stacks using a nano-positioning stage. But I don't know how to analyse that data to calculate the psf. I have got an ImageJ plugin PSFGenerator. Can I find some sort of user...

05 June 2018 5,501 3 View

How do I check for successful coating of CYTOP on glass slides?

I need to deposit layers of CYTOP on glass slides and I plan to use spin coating method. How do I make sure the layers have formed a uniform coating?

02 March 2018 6,362 2 View

How can I prepare sample for SEM, TEM, and XRD?

I have a solution of magnetic nanoparticles that I need to characterise. How do I proceed?

01 February 2018 443 13 View

What do you man by silver island films(SiFs), in the context of metal enhanced fluorescence?

I need to understand the structure of SiFs.

31 December 2017 396 0 View

I'm looking for any article that best sums up the current understanding we have on metal enhanced fluorescence (MEF) and microwave accelerated MEF?

Can anybody point out any paper or letter or editorial or anything that can give an overview of the present know-how as well as where the fields of MEF and MAMEF are heading?

31 December 2017 469 1 View

Can somebody explain this particular paragraph on surface plasmon resonance?

"The mean free path in gold and silver is ~50 nm, therefore in particles smaller than this, no scattering is expected from the bulk. Thus, all interactions are expected to be with the surface....

11 December 2017 4,612 3 View

Fluorescence assays: limit of detection

what are the current achievable standards in terms of lower detection limit in fluorescence based assays?

01 January 1970 1,895 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View