Arti Tyagi

10 Questions 35 Answers 0 Followers

Questions related from Arti Tyagi

Why does my RT-qPCR reaction of microRNA shows amplification only after 40 cycles?

I am trying to construct a standard plot for miR21 and miR 200 (Cq vs conc.) using synthetic oligos but even on using miRNA concentrations of upto 100pM I only begin to get any amplification...

09 September 2019 6,036 9 View

Does the particle size reported with AFM images actually mean their height? Or is it the diameter?

I have tried depositing metal colloidal films on glass slides (paper attached). They reported particles sizes using AFM image analysis which is very small compared to what I have got. It could be...

02 February 2019 2,036 11 View

I need to experimentally determine the psf of my microscope using fluorescent beads?

I have captured the z-stacks using a nano-positioning stage. But I don't know how to analyse that data to calculate the psf. I have got an ImageJ plugin PSFGenerator. Can I find some sort of user...

06 June 2018 5,607 3 View

What is the difference between the various homologs of a single coenzyme?

I was looking for CoQ10 ELISA kits and I came across some labelled as 'CoQ10 binding protein homolog A, mitochondrial' or CoQ10... homolog B..' what is the difference between these two in terms...

04 April 2018 574 5 View

How do I check for successful coating of CYTOP on glass slides?

I need to deposit layers of CYTOP on glass slides and I plan to use spin coating method. How do I make sure the layers have formed a uniform coating?

03 March 2018 6,407 2 View

How can I prepare sample for SEM, TEM, and XRD?

I have a solution of magnetic nanoparticles that I need to characterise. How do I proceed?

02 February 2018 521 13 View

What do you man by silver island films(SiFs), in the context of metal enhanced fluorescence?

I need to understand the structure of SiFs.

01 January 2018 451 0 View

I'm looking for any article that best sums up the current understanding we have on metal enhanced fluorescence (MEF) and microwave accelerated MEF?

Can anybody point out any paper or letter or editorial or anything that can give an overview of the present know-how as well as where the fields of MEF and MAMEF are heading?

01 January 2018 522 1 View

Can somebody explain this particular paragraph on surface plasmon resonance?

"The mean free path in gold and silver is ~50 nm, therefore in particles smaller than this, no scattering is expected from the bulk. Thus, all interactions are expected to be with the surface....

12 December 2017 4,663 3 View

Fluorescence assays: limit of detection

what are the current achievable standards in terms of lower detection limit in fluorescence based assays?

01 January 1970 1,976 7 View