Why does my RT-qPCR reaction of microRNA shows amplification only after 40 cycles?

I am trying to construct a standard plot for miR21 and miR 200 (Cq vs conc.) using synthetic oligos but even on using miRNA concentrations of upto 100pM I only begin to get any amplification...

08 September 2019 5,927 9 View

Does the particle size reported with AFM images actually mean their height? Or is it the diameter?

I have tried depositing metal colloidal films on glass slides (paper attached). They reported particles sizes using AFM image analysis which is very small compared to what I have got. It could be...

01 February 2019 1,951 11 View

I need to experimentally determine the psf of my microscope using fluorescent beads?

I have captured the z-stacks using a nano-positioning stage. But I don't know how to analyse that data to calculate the psf. I have got an ImageJ plugin PSFGenerator. Can I find some sort of user...

05 June 2018 5,501 3 View

What is the difference between the various homologs of a single coenzyme?

I was looking for CoQ10 ELISA kits and I came across some labelled as 'CoQ10 binding protein homolog A, mitochondrial' or CoQ10... homolog B..' what is the difference between these two in terms...

03 April 2018 515 5 View

How do I check for successful coating of CYTOP on glass slides?

I need to deposit layers of CYTOP on glass slides and I plan to use spin coating method. How do I make sure the layers have formed a uniform coating?

02 March 2018 6,362 2 View

How can I prepare sample for SEM, TEM, and XRD?

I have a solution of magnetic nanoparticles that I need to characterise. How do I proceed?

01 February 2018 443 13 View

What do you man by silver island films(SiFs), in the context of metal enhanced fluorescence?

I need to understand the structure of SiFs.

31 December 2017 396 0 View

Can somebody explain this particular paragraph on surface plasmon resonance?

"The mean free path in gold and silver is ~50 nm, therefore in particles smaller than this, no scattering is expected from the bulk. Thus, all interactions are expected to be with the surface....

11 December 2017 4,612 3 View

Fluorescence assays: limit of detection

what are the current achievable standards in terms of lower detection limit in fluorescence based assays?

01 January 1970 1,895 7 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View