For XRD you need powder. What is more, if you can, dont use copper tube, bcs you will obtain background from fluorescence of Fe3O4.

For SEM: Use copper foil as substrate, Drop on it Fe3o4 disspersion, dry and next you must deposit on it Ag, Pd or Au conduction layer.

For TEM: You must use disperrsion with very low concentration in EtOH. Then drop one-two drops on the copper grid with carbon layer , dry in ambient temperature and then you can analyse nanopartciles.

If you want, you can read my article about characterization of modified magnetite nanoparticles :).

Article Structure and optical properties of Fe 3 O 4 nanoparticles s...

Best regards.

How do you need to characterize them - size? Composition?

You may find this page useful: https://nanocomposix.com/pages/characterization-techniques

If you only need a measure of the particle size, you can use UV-vis absorption spectroscopy, which can be done with a solution (edit: I meant to say suspension). You need a dry sample for SEM and TEM (as they're carried out in vacuum), so you need to deposit the nanoparticles on something before characterization. The above page gives basic information for TEM.

For XRD you need powder. What is more, if you can, dont use copper tube, bcs you will obtain background from fluorescence of Fe3O4.

For SEM: Use copper foil as substrate, Drop on it Fe3o4 disspersion, dry and next you must deposit on it Ag, Pd or Au conduction layer.

For TEM: You must use disperrsion with very low concentration in EtOH. Then drop one-two drops on the copper grid with carbon layer , dry in ambient temperature and then you can analyse nanopartciles.

If you want, you can read my article about characterization of modified magnetite nanoparticles :).

Article Structure and optical properties of Fe 3 O 4 nanoparticles s...

Best regards.

Thank you Adrian, but my particle size is huge, ~100nm, will that pose a problem?

For SEM you can use double-sided carbon tape.

Use FESEM for better resolution and be care full while sticking on double side adhesive carbon tape. Find attachment for further information

for PXRD try to calcinate at higher temperature.

For TEM analysis, make your sample dispersion by take a least quantity of your sample dispersed in a epoxy resin (Gatan Inc.) and drop-casting two or three drops onto glass slide and spread it like a thin sheet using spin coater or manually by using glass rod. Then, allow to dry at room temperature overnight. Finally peel of the thin sheet from the glass slide and cut it like a circular disc of 3mm diameter using scissor or punching machine and then fix it on a TEM sample holder.

So that, we can avoid our samples to stick it on an electromagnetic lenses in the TEM equipment.

For SEM, the same thick sheet can be used. Gold coating is required for SEM analysis for better image resolution.

For XRD, you can use your as such nano particles.

All the best.

you can make powder of your sample for SEM & X-RD using some basic methods and you can get your sample characterized by SEM by making thin film using various processess. for TEM you are required to make a dilute dispersion of the sample. (see my paper if needed or contact me at [email protected])

For XRD measurement as prepared sample is enough to measure the data.

Make sure the sample uniformly distributed on the XRD sample holder.

2.For SEM sample preparation there is two ways.

1. Small amount of sample coated on the carbon tape.

If it is conductive you can measure the SEM data.

If it is non -conductive make carbon coating on the surface.

otherwise sample get charge you cannot view the image.

3.For TEM sample preparation you need to dissolve the sample in ethanol and take one or two drops on the copper grin and anneal 50C to evaporate the ethanol.

Make sure the handling of the copper grid its very sensitive.

Hope this will help you.

For both SEM and TEM characterzation, solution can be prepared by adding 0.1mg sample in 1ml distilled warer and sonicate for 10-15 min.

For SEM and TEM characterization the basic difference is of actually deposition concentration on the grids. TEM needs more attention. For both firstly dilute the powder in the solvent and sonicate to make uniform solution, then deposit on the required grids make them dry

Dear Arti,

I suggest you to read the followings. You will find all of your answers.

Article Antimicrobial potential of green synthesized CeO2 nanopartic...

Article Green-synthesised cerium oxide nanostructures (CeO2-NS) show...

-ALL the BEST-

fresh surface was very enough for SEM