I need to understand the structure of SiFs.
I am trying to construct a standard plot for miR21 and miR 200 (Cq vs conc.) using synthetic oligos but even on using miRNA concentrations of upto 100pM I only begin to get any amplification...
08 September 2019 5,927 9 View
I have tried depositing metal colloidal films on glass slides (paper attached). They reported particles sizes using AFM image analysis which is very small compared to what I have got. It could be...
01 February 2019 1,951 11 View
I have captured the z-stacks using a nano-positioning stage. But I don't know how to analyse that data to calculate the psf. I have got an ImageJ plugin PSFGenerator. Can I find some sort of user...
05 June 2018 5,501 3 View
I was looking for CoQ10 ELISA kits and I came across some labelled as 'CoQ10 binding protein homolog A, mitochondrial' or CoQ10... homolog B..' what is the difference between these two in terms...
03 April 2018 515 5 View
I need to deposit layers of CYTOP on glass slides and I plan to use spin coating method. How do I make sure the layers have formed a uniform coating?
02 March 2018 6,362 2 View
I have a solution of magnetic nanoparticles that I need to characterise. How do I proceed?
01 February 2018 443 13 View
Can anybody point out any paper or letter or editorial or anything that can give an overview of the present know-how as well as where the fields of MEF and MAMEF are heading?
31 December 2017 469 1 View
"The mean free path in gold and silver is ~50 nm, therefore in particles smaller than this, no scattering is expected from the bulk. Thus, all interactions are expected to be with the surface....
11 December 2017 4,612 3 View
what are the current achievable standards in terms of lower detection limit in fluorescence based assays?
01 January 1970 1,895 7 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View
Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...
20 February 2021 1,291 3 View
I'm using blast furnace slag for stabilizing subgrade soil.
17 February 2021 7,333 3 View
Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...
16 February 2021 453 2 View
