I need to understand the structure of SiFs.
I am trying to construct a standard plot for miR21 and miR 200 (Cq vs conc.) using synthetic oligos but even on using miRNA concentrations of upto 100pM I only begin to get any amplification...
08 September 2019 6,103 9 View
I have tried depositing metal colloidal films on glass slides (paper attached). They reported particles sizes using AFM image analysis which is very small compared to what I have got. It could be...
01 February 2019 2,100 11 View
I have captured the z-stacks using a nano-positioning stage. But I don't know how to analyse that data to calculate the psf. I have got an ImageJ plugin PSFGenerator. Can I find some sort of user...
05 June 2018 5,682 3 View
I was looking for CoQ10 ELISA kits and I came across some labelled as 'CoQ10 binding protein homolog A, mitochondrial' or CoQ10... homolog B..' what is the difference between these two in terms...
03 April 2018 623 5 View
I need to deposit layers of CYTOP on glass slides and I plan to use spin coating method. How do I make sure the layers have formed a uniform coating?
02 March 2018 6,447 2 View
I have a solution of magnetic nanoparticles that I need to characterise. How do I proceed?
01 February 2018 567 13 View
Can anybody point out any paper or letter or editorial or anything that can give an overview of the present know-how as well as where the fields of MEF and MAMEF are heading?
31 December 2017 562 1 View
"The mean free path in gold and silver is ~50 nm, therefore in particles smaller than this, no scattering is expected from the bulk. Thus, all interactions are expected to be with the surface....
11 December 2017 4,703 3 View
what are the current achievable standards in terms of lower detection limit in fluorescence based assays?
01 January 1970 2,018 7 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
We are writing an essay on climate change and its impact on indigenous people circumpolar north people, native nations, islandic, aborigenal of pacific and so on
16 July 2024 5,248 3 View
what all details we will get from fluorescence life time data
10 July 2024 505 1 View
I am a beginner in this field. While I am reading papers and the understanding is getting better. However can someone explain in easy and simple words. Really thankful !
08 July 2024 8,337 5 View
