Hello.
I need to know the composition of chondrogenic medium. Can we use 10%FBS along with 1%ITS in chondrogenic medium?
If possible can somebody give me reference article in which both of these components are used together for chondrogenic induction.
Thanks in advance.
Hi
Complete media (low glucose DMEM with 10% FBS, l-glut and pen/strep) supplemented with 10ng/ml TGFbeta 1/3, insulin-transferrin-selenium x supplement premix (ITS premix). This was a 21 day protocol, which did not contain dexa, and was always successful as shown using alcian blue and safranin-o tinctorial staining, as well as collagen II and aggrecan expression.
Human ADSCs and induce chondrogenesis using a protocol from Guasti L et al., Stem Cells Transl Med, 2012. This protocol contains 0.1uM dexa and 1x ITS (life tech premix).
Hope this helps
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