Hi,
Anybody knows how can we quantify total amount of specific protein i.e X protein from western blot of cytosolic and nuclear fractions?
Thanks in advance. Best.
You could use a densitometer or any available gel analysis software or antibody staining where the antibody is labelled with a fluorophore or some other technique.
if you want to know the absolute amount you may also load some purified protein in a known amount as your 'marker' then you can compare the densitometry.
Thanks V.N. Ravi Kishore V.
@Yuning Hou. That is a nice idea. Thank you so much.
Best
Instead of quantifying your protein with respect to one standard protein of known amount/concentration. Create a calibration curve and use it to quantify your protein, the accuracy is better.
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