Sajjad Ashraf

4 Questions 6 Answers 0 Followers

Questions related from Sajjad Ashraf

Does anybody has Crisper dCas9 gRNA Plasmid having following components?

Hi, I am looking for a Plasmid containing following properties: 1. dCAS9 sequence 2. gRNA scaffold 3. U6 or T7 promoter sequence 4. Piggy bag backbone 5. Puromycin or Hygromycin selection...

08 August 2018 3,803 0 View

How to quantify total protein in the cell from western blot of Cytosolic and nuclear protein?

Hi, Anybody knows how can we quantify total amount of specific protein i.e X protein from western blot of cytosolic and nuclear fractions? Thanks in advance. Best.

09 September 2016 3,661 4 View

What is the composition of Chondrogenic medium?

Hello. I need to know the composition of chondrogenic medium. Can we use 10%FBS along with 1%ITS in chondrogenic medium? If possible can somebody give me reference article in which both of these...

12 December 2015 2,665 2 View

Does anyone have experience with controls for nuclear protein?

Can I run GAPDH as a control in western blotting for nuclear protein fraction? Thank you.

03 March 2015 3,690 8 View