If opposite charges attract each other, then why do neutrons and protons bind with each other, whereas electrons surround them in an atom?

According to science, like charges repel each other and opposite charges attract each other. But if you observe the structure of an atom, neutrons(neutral), and protons(positive) bind tightly with...

23 November 2020 8,881 7 View

What are the effects of chemical fertilizers on human health ?

Can any fertilizer causes cancer ?

06 September 2020 8,217 11 View

How can one determine the lifetime of a compound if quantum yield is known?

I determined the quantum yield of my compound from fluorescence instrument. So can i determine the total lifetime of my compound from this data?

19 August 2020 521 1 View

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04 July 2020 9,111 0 View

How can i ensure the complete oxidation of graphite before quenching the oxidation process by H2O2?

I am following KMnO4 -H2SO4 reaction media for the synthesis of graphene oxide(GO) by an improved process.

04 June 2020 508 3 View

Can anyone help me to know whether band gap of a semiconductor can be changed by loading dye sensitizer or not?

1- n-type semiconductor has an absorption spectra 2-Dye has an absorption spectra 3- Semiconductor+ Dye has an absorption spectra 4- Can I calculate the band gap of of SC, Dye and SC+Dye using...

27 March 2020 4,222 4 View

What is a multi-label classification and how to use it on classifying text documents?

I want to know how multi-label classification work in detail. At the same time, how does the confusion matrix for multi-label classification is constructed? How does the performance measures such...

28 December 2019 8,065 3 View

What are the Model fit parameters for multigroup analysis in AMOS?

I have used SEM with AMOS for my data for hypothesis testing and the model parameters came out good. I wanted to conduct a multigroup analysis with the same sample by categorizing them into two...

16 December 2019 7,453 3 View

Can I use XBridge C18 ( 3x150mm, 3.5 um) column in a Aquity UPLC system for method development?

I have a UPLC system and XBridge C18 ( 3x150mm, 3.5 um). I am running short of time and cant procure a UPLC compatible column for the time being.

15 December 2019 8,433 12 View

How many different origin of replication (ori) sequences can I have in a plasmid?

Multiple ori sequences pertaining to different hosts are usually present in a plasmid. But how many different ones can I have, without causing problems? If problems do get created, what kind of...

25 November 2019 400 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

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28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View