Hi,
I have only worked with tissue sections stored in RNA later. To pellet down the sections you have to change the density of the liquid. It seems that cells stored in RNA later has a similar density as the buffer itself. I add the same volume of RNase-free water and immediately centrifuge the tubes. I think this will also work for cell suspensions... good luck
hii Aditya,
May I know how much centrifugal force and CF time you used to pellet out your cell sample???
go through this link this will be definitely helpful for you ....http://zh.invitrogen.com/etc/medialib/en/filelibrary/Nucleic-Acid-Purification-Analysis/PDFs.Par.18819.File.dat/bp_7020.pdf ...
good luck ...
Thanks Norman and deepa for your suggestion. I have centrifuged from low speed to upto 13000rpm for pelleting but no pellet precipitated. I have gone through invitrogen protocol before but they didnot mention about this problem. They only talked about pelleting. I will have to try upon norman's idea and will see.
Thanks all for your suggestion.
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