If two cells are of different densities, can they be separated through density gradient centrifugation? or any other method? Similarly if two human cell lines are of different sizes can they be separated by using some filters?
The separation by centrifugation using density gradient is good method to isolated subpopulations but not ideal. Resulted population purity will vary strongly depending of cellular features. If purity of 90-98 % will be suiable for your goal then try to do it. This level could be reached if cells don't aggregate.
Thanks Daniil for your answer. Do you have idea if cells can be grown after separation by density gradient method?
It is usual situation for lymphocytes to grew them after separation in Ficoll gradient. As far you will keep physiological non-cytotoxic conditions it possible to use it after. What kind of cells do you want to separate?
want to seperate polyploid breast cancer cells (human) from non-polyploid cells in a mixed population. Expects polyploid cells to be of higher density.
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