What is the best TGFb-inhibitor to use in cell culture experiments: SB431542 or LY-364947?

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How do you feel about social exclusion in transport within South Africa?

Research being conducted by Stellenbosch University would like your opinion about the effects of social exclusion on emerging urban mobility options and traditional public transport services. By...

21 July 2024 4,032 0 View

Calculating the injection volume for a mouse?

I will be administering 5-fluorouracil at a dosage of 50 mg/kg to mice as an I/P injection. Will someone please check my calculation? (Mw: 130.078 g/mol) Max volume to be administered to mice I/P...

23 June 2024 3,847 1 View

Finding Survey Participants?

Dear Survey Experts, I am currently researching trust and resilience in the supply chain and seeking efficient ways to find survey respondents. In addition to social media and business...

21 June 2024 395 2 View

How to interpret meta-analysis of incidence rates?

Hi! I have a question. I was looking into the research question: does mohs micrographic surgery give less recurrences and metastasis than standard excision in the treatment of cuteanous squamous...

17 June 2024 7,769 5 View

How to functionalise the surface of a PMMA microparticle with amines?

I have porous PMMA microparticles up to 1mm in diameter. I would like to functionalise the surface with amines whilst keeping the particles intact. Chat GTP has recommended ethanolamine or ATPES...

13 June 2024 7,977 1 View

How to avoid metal contamination from a sonicator?

Hi, I have been using a Q-Sonica Q500 Sonicator to disperse samples on nanocellulose and make emulsions. I have notices black dust like particulates settling out in some samples and on the bottom...

12 June 2024 9,737 6 View

How to calculate the conversion of random copolymerization mixture?

I'm working on the RAFT copolymerization of MPC and NIPAm monomer, but I'm not sure how to calculate the total conversion from proton NMR? Is it an average of conversions of two monomers or just...

10 June 2024 7,430 2 View

Can I or you add publications to the page of Adri Jacobs?

Dear ResearchGate, I came across the ResearchGate page of Adri Jacobs, who passed away in 2010. This page contains 25 publication of Adri mainly from the period 2000-2008. One paper (2011) must...

01 June 2024 8,763 1 View

Lifestyles and correlations in cultural tourism?

I am looking for resources in the Netherlands regarding cultural tourism. So what cultural activities correlate, and what lifestyles can be distinguished. Any resource is welcome, articles, factor...

27 May 2024 447 2 View

How do I get rid of the background signal using the Bio-Rad Quantity One?

I've coated plastic water bottles (PET) with Nile Red to detect the plastic degradation by Ideonella sakaiensis. When using the settings for Sypro Ruby, the coated and uncoated plastic looks the...

23 May 2024 3,364 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View