I want to store some feces samples for bacteria isolation. I am planning to store them in -800C. Will it be ok? How long can I store that way without destroying the bacteria?
Hello.
Everytime I had to isolate bacteria from faeces I did it right after taking samples...
It all depends what is your answer. If you want to know the actual population I recomend you procesing samples quickly after sampling. After samples are taken microbial community in faeces will change, this changes will depend on time and temperature.
As a matter of "getting to the lab" a refrigeration procedure it will be fine, you will be able to delay growth
Freezing it is a good way to preserve "what was there" but... if you want to isolate I believe that many of the bacteria would have die at the isolation time.
Anyways, I have read some papers where they stored faces at -80 and still recovered bacteria, again, the thing is what you want to answer. If you only want to isolate some particular group without a quantitative aim freezing will be ok.
Thank you very much for your answers. I think the processing of fresh samples seems to be the best method.
Hello Mohan.
yes, if you want to have a better "picture of reallity" processing fresh samples it is the best. The more you process and wait, the more things will change.
to you orders at any time.
You may choice between process the feces immediately, or to take a swab of a representative part of the feces and preserve it in a transport medium (Cary Blair) at room temperature
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Microorganisms
- Bacteria