I want to develop Tamoxifen resistant cells for my research but getting confused with different protocol used.
See the manuscript:
Farabegoli F., Papi A., Bartolini G., Ostan R., Orlandi M., (-)-epigallocatechin-3-gallate downregulates Pg-P and BCRP in a tamoxifen resistant MCF-7 cell line, Phytomedicine, 17 : 356-362, 2010.
Also check this manuscript:
Leung, E., Kannan, N., Krissansen, G. W., Findlay, M. P., & Baguley, B. C. (2010). MCF-7 breast cancer cells selected for tamoxifen resistance acquire new phenotypes differing in DNA content, phospho-HER2 and PAX2 expression, and rapamycin sensitivity. Cancer biology & therapy, 9(9), 717-724.
For my in vivo work I always dissolved tamoxifen in oil (such as corn or sunflower oil). However for in vitro experiments we use 4-hydroxytamoxifen, the active metabolite of tamoxifen. This compound dissolves quite well in ethanol.
Hi Ebidor,
I agree with Jaroslav, it is vital to use 4-hydroxytamoxifen (afimoxyfene), the active metabolite, as Tamoxifene will be inactive in cell culture. You can also make 5mM (2mg/ml) Stock in DMSO.
Good Luck
Thanks all but I just realised I got tamoxifen citrate instead of 4-hydroxyltamoxifen and was wondering if I could still use it. has anyone tried it in invitro experiment?
Hi Ebidor,
If you solubilize the tamoxifen citrate you will get a tamoxifen solution. Which is not suitable for in vitro - see above. If you have problems getting hold of the hydroxy, please let me know, I can dig up our supplier.
Hi Ebidor,
I confused this with another active metabolite of another drug (Ifosfamide) which is actually kind of hard to get hold of.
But the 4-hydroxy Tamoxifen is ubiquitously available. We got ours from cayman chemicals, but you will also find it at sigma, tocris, abcam, scbt etc. etc.
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