I read I could use water, dmso, ethanol 100%. DMSO and ethanol seem to be better solvents than water but add a supplemental stress to cells. Do you have any advice?
I have been using cycloheximide for polysome analysis for years. Have always used it by dissolving in water and have had no problems for dissolving up till 10mg/ml of water.
According to Sigma, it is soluble in water to 20 mg/ml (with sonication) and to 5 mg/ml (=17.7 mM) easily. It is quite stable at acidic pH, especially frozen (for long-term storage): https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/c7698pis.pdf
In terms of absence of the solvent after the addition, ethanol can be also convenient (up to 100-200 mM stocks: https://www.selleckchem.com/products/cycloheximide.html). You can place a small volume of a concentrated stock in a tube/vessel (e.g. 1 ul / 10 ml for 1x, 1 ul / 1 ml for 10x solution), evaporate it (happens quickly, especially under vacuum) and then mix with a water-based media to redissolve.
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