12 Questions 117 Answers 0 Followers
Questions related from Michael Koksharov
Is there a SMILES notation to properly reflect chirality of the bonds in the chemical compounds corresponding to pentavalent transitions states?
23 July 2025 8,516 1 View
What is the thickness of adherent HEK293, HEK293T cells growing on cell culture plates? The commonly cited value of average cell size (diameter) for spherical HEK293 cells is 11-15 μm...
27 April 2025 5,633 1 View
Refined oils from plant seeds are mixtures of triacylglycerols (TAGs), up to 99%, but they still contain some amount of other lipids and small molecules. In general, edible plant oils contain 98%...
26 February 2025 5,086 2 View
There are theories that petroleum can in some cases form in an abiogenic way (though, most of the Earth's petroleum oil seems to come from remains of living organisms). Are there some indications...
17 December 2024 2,774 1 View
It's not a secret that while truly pure imidazole does not have absorbance above 260 nm, typical imidazole products on the market (such as ACS, "Ultrapure", etc grades) do have absorbance of about...
10 May 2023 4,901 3 View
Which Gymnocalycium species can cross between each other? As I understand, most Gymnocalycium do not self-pollinate. But which ones can be crossed between each other? In nature and in cultivation.
27 March 2023 1,500 1 View
What are the differences between PTFE and ETFE tubing (0.5-1 mm inner diameter) when used in protein chromatography purifications? Both polymers are used to make small diameter transparent tubings...
07 February 2023 2,103 7 View
Why so many restriction enzymes have a very different activity (from very low to high) in NEB Buffer 2 vs NEB CutSmart buffer...
17 February 2019 7,953 0 View
Who was Ten Broeck after whom the eponymous tissue homogenizer (Ten Broeck or Tenbroeck) is named? For Dounce there is a Wikipedia page (https://en.wikipedia.org/wiki/Alexander_Dounce). But...
27 October 2017 7,791 1 View
The lifetime (gradual or induced/regulated) degradation of cytoplasmic and nuclear proteins is usually mediated by ubiquitin-proteasome system. What happens with proteins that are inside...
15 October 2017 3,805 1 View
Usually it is expected that researchers should make available all the reagents used/studied in their publication unless they are commercially available. Recently one author refused to send me a...
19 February 2017 10,077 2 View
It is usually possible to screen libraries of drugs, siRNA, reporter vectors in the same cell line. But if one wants to compare the transcriptional activation of the same reporter (or an...
23 January 2017 4,021 1 View
