Sabarish Nagarajan

3 Questions 25 Answers 0 Followers

Questions related from Sabarish Nagarajan

What would be a good positive control for proteins associated with polyribosomes in Drosophila?

I am working on Drosophila melanogaster and would like to know what would be a simple/easy protein that is known to associate with polysomes. The point of my experiment is to do polysome analysis,...

06 October 2015 2,149 3 View

Has ribophagy been investigated in Drosophila or any other model organism?

I am interested in changes in mRNA translation and was wondering if changes in mRNA translation are affected by changes in ribosome numbers. I see decrease in 18S and 28S rRNA in Drosophila after...

04 November 2014 7,925 2 View

What is the association of mRNA with ribosomes?

When I do qRT_PCRs in my polysome fractions of Drosophila larval lysates, I see around 95% of a particular mRNA (eg. actin) associated with 2 or more ribosomes. Why is it that ribosome association...

20 September 2014 858 10 View