Benoit Besson

4 Questions 4 Answers 0 Followers

Questions related from Benoit Besson

Quick cell lysis by osmotic shock

Hi everyone, We are looking for a quick/instant method to perform cell lysis (HEK cells) while monitoring luciferase activity from t=0 to t=5-30 min let say. So the lysis buffer need to be very...

21 June 2017 3,138 7 View

Has anyone encountered poorly efficient smartpooled siRNA?

Hi everyone, I performed an assay following a protocol already established in the lab for a single homemade siRNA: 6 µL Dharmafect 1 mixed with siRNA in 200 µL serum free / antibiotic free medium...

06 May 2016 7,869 4 View

What is the best solvent to dilute cycloheximide for cell application?

I read I could use water, dmso, ethanol 100%. DMSO and ethanol seem to be better solvents than water but add a supplemental stress to cells. Do you have any advice?

01 April 2014 9,043 3 View

Protein expression problem in mammalian cell line?

I try to over-express some proteins with a cMyc tag in mammalian cell lines (HeLa at least). I've inserted by PCR a CAT gene with a cMyc tag into the plasmid pcDNA3.1(-) with a kozak sequence...

15 January 2014 6,198 3 View