Today I installed GraphPad Prism to estimate IC50 and I have absorbance and concentration values but really do not how to estimate as first time to use it and I'm so confused.
I've also tested GraphPad Prism and Origin, which are good software.
As something different, I'd check out thunor (https://www.thunor.net/). This is free software, based on a python library (https://github.com/alubbock/thunor).
As soon as you start doing high-throughput dose-response analyses, it's worth learning R or python and setting up your own automation. Thanks to the online courses available these days, this is easier than you think. In the end, it'll probably be faster than trying to get publication-quality data from excel.
For high-throughput analysis we developed our own python library, ECCpy (https://github.com/teese/eccpy). ECCpy allows you to automatically and objectively exclude bad data, by defining parameters for quality control.