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Questions related from Bashir Alsiddig Yousef
We use to use FBS form Gibco to culture our colon cance cell lines (HCT-116, COLO-205, HT-29 and SW-620) and it were o.k, with good morphological appearance as in the database, I sub-cultured them...
26 November 2014 9,517 5 View
I am intending to use TGF-beta receptor 1 antibody as a blocker in cultured fibroblasts treated with TGF-b. In general, to assess the FMT (fibrobllast-to-myofibroblast transition), cells are...
10 June 2014 4,475 2 View
Sample denaturation is one of the steps that should be carried to prepare the proteins for SDS-PAGE. Should we carry this step out for all proteins? Or can some proteins be affected by this...
12 March 2014 6,860 13 View
I am using colon cancer cell (COLO-205), which is a mix-type of cell (suspension and attached), if I take the media and centrifuge it and then I resuspend the cell with media, and then I add it...
31 May 2013 8,297 6 View
I am using culture media to dissolve my drug as the drug is not stable in DMSO, so I am keeping fresh drug solution for when I want to carry any experiment (cytotoxicity, cell cycle analysis,...
23 May 2013 5,902 7 View
I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as...
06 May 2013 7,053 6 View
Does anybody have any idea about how the mTOR pathway can affect apoptosis? Or what is the kind of relationship between these 2 cellular pathways?
25 March 2013 8,361 2 View
