We have both paraffin- waxed tissue samples and blood from patients We would like to know what is the best method to use for the analysis of EBV?
There is a recently published article using EBV DNA viral load for the blood samples.
We stain mRNA of EBV (EBER) in paraffin section with a commercial kit (CISH). It works well, but is only a yes/no analysis. I have no infos about comparison to sensitivity of blood tests.
Real time PCR works well on both type of samples, with FRET, you just have to prepare the paraffine samples before extraction of DNA.
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
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