Marc-Antoine Perrenoud

9 Questions 17 Answers 0 Followers

Questions related from Marc-Antoine Perrenoud

AAV Extraction from supernatant ?

I am trying to perform AAV long-read sequencing using the PacBio protocol. The protocol requires 1.5x10e12 Vg input to use 100uL and get 1ug DNA at the end of extraction, in order to get around...

19 October 2023 738 1 View

DNA extraction M-N QuickPure on Buccal swabs ?

As we are performing method validation for our DNA extraction protocol we are facing issues. Sampling Procedure : Inside of the cheek, swab with a little bit of strenght 10-15 secondes while...

27 July 2022 8,629 0 View

Plasmid and Positive controls conservation ?

Hey, In order to standardize and optimize positive controls for Real-time PCR in our laboratory, and also to gain time, we were wondering the stability of plasmid dilutions for semi to long term...

10 March 2017 8,942 3 View

How do I transform RNA into cDNA in a 20minutes reaction or less?

I'm looking to improve the workflow inside my laboratory. I would like to be able to transform RNA (extracted from patients samples, respiratory viruses, Hepatitis E and D). our current reaction...

19 January 2016 3,597 2 View

Any advice on the standard for Colony PCR on direct samples ?

Hi Folks, We performed colony PCR on 16S eubacterial gene for a long time, since a bit of time we got small issues with our positive controls, the band aren't so bright and beautiful as before. We...

21 December 2015 9,365 8 View

How can I increase the performance of my Eubacterias PCR on Patients sample?

I explain, i'm doing PCR on Chlamydia trachomatis. The PCR on strains works fine, i got large bands on gel, and when i'm sequencing it, the result is perfect.Then i had to do the PCR on patients...

24 February 2015 2,815 4 View

Does anyone have any knowledge of PCR inhibitions by Prepman extraction vs MagNA Pure Extraction?

I'm currently running PCR for fungal (18S gene, 26S gene). My PCR for 18S and 26S works fine on fungi (extracted with MagNA Pure 96 from Roche), but I can't get amplification for yeast...

20 February 2015 629 1 View

Does anyone know which kit is the best for large spectre PCR (identifiactions Eubacterias, mycobacterias, resistance genes TB)?

I'm starting my tests next week, I will test 4 different kits from 4 brands and I desire to know if anyone has tested FastPCR for diagnosis purpose with one of those ready-to-use pcr mixes? I...

29 December 2014 6,193 4 View

Does anyone have knowledge of the efficacity of TaKaRa Ex Taq Hot-Start Version kit in large spectre PCR for diagnosis?

I'm trying to optimize a polymerase chain reaction for detection of eubacterias, mycobacterias, among other things. I'm trying several different kits and I wanted to know the pros/contras of this...

12 November 2014 1,922 3 View