Hello!
I am planning to culture whole human bone marrow for 3 to 4 days and need a culture to maintain all the possible cell types, including mesenchymal stroma cells and their fibroblasts, etc. as well as HSC.
However, there are many commercial mediums available and I am a bit confused because there are too many cultures for different purposes. For instance, there is a culture for growing myeloid cells, another for stromal cells, and another for mesenchymal stromal.
So I decided to make a cocktail consisting of all of these mediums to keep everyone happy in the culture.
But I am wondering if there is a medium that contains different substances that maintain the growth of all the cells in human bone marrow??
I found one from ThermoFisher (Gibco) but not sure if it is good for all the cells, as the manufacturer just mentioned good for human stromal cells and contains a blend of hematopoietic growth factors.
Thank you!
Hello Sina Tadayon
Why don’t you try McCoy's 5a medium supplemented with fetal bovine serum, horse serum, and hydrocortisone. For more information you could refer to the article I have attached below.
Article Gartner S, Kaplan HSLong-term culture of human bone marrow c...
Best.
Ask your self, why you want to culture bone marrow for 3-4 days. There is no one-size-fits-all medium that will maintain the growth of all cell types in human bone marrow, as different cell types have distinct growth requirements. However, there are several commercially available culture media that can be used to support the growth of a variety of cells present in human bone marrow, including mesenchymal stromal cells (MSCs) and hematopoietic stem cells (HSCs).
A commonly used medium for culturing human MSCs is an alpha-minimum essential medium (α-MEM) supplemented with 10-20% fetal bovine serum (FBS) and a mixture of antibiotics to prevent bacterial contamination. Another commonly used medium for MSCs is Dulbecco's Modified Eagle Medium (DMEM) supplemented with the same components.
For culturing HSCs, a serum-free medium such as StemSpan SFEM II is often used, which contains a combination of growth factors and cytokines to support the growth of HSCs. Alternatively, culture media such as IMDM or RPMI 1640 can be used with the addition of cytokines, such as erythropoietin (EPO), granulocyte-colony stimulating factor (G-CSF), and thrombopoietin (TPO).
This is just a note to say that in the oxygen concentration [O2] is usually less than 10 micro molar in the bone marrow, and culturing these cells under the usual O2 concentrations used in tissue culture will likely stress them considerably.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line