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Hello! I am planning to culture whole human bone marrow for 3 to 4 days and need a culture to maintain all the possible cell types, including mesenchymal stroma cells and their fibroblasts, etc....
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Hello everyone! I am working with DsRed and GFP expressing mice and noticed that there are different cells with different expression levels of DsRed or GFP in a same tissue. For instance, I...
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Hello everybody, I'm looking for a tumor cell line (B16 or LLS) expressing OVA protein on the cell surface that I can use with OT I & OT II T cells. Does anyone have them so can share with...
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Hello Hello, I am trying to stain DsRed/GFP lymphocytes in recipient tissue. Tissues are frozen spleen embedded in OCT gel. I used a primary Rabbit polyclonal to RFP (Anti-RFP antibody ab62341)...
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I have generated DC from mice bone marrow. I have followed very known protocol, incubating with GM-CSF for 8 days and LPS activation overnight or 48 hours. After LPS activation, I stained my...
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I am working with adaptively transferring leukocytes in tumor-bearing mice. So I am taking the cells from the donor animals, label them with fluorescent dyes and inject them back to the...
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