11 Questions 16 Answers 0 Followers
Questions related from Sina Tadayon
Hello! I have isolated Lin- CD117+CD150+Sca1+. But there are two types of cells here: CD48+ and CD48 -. What is the difference between them? Can I call CD48+ cells more differentiated HSC and...
15 November 2023 5,935 2 View
Hello! I am planning to culture whole human bone marrow for 3 to 4 days and need a culture to maintain all the possible cell types, including mesenchymal stroma cells and their fibroblasts, etc....
10 February 2023 8,442 3 View
Hello! I am phenotyping the mouse bone marrow, and I only need to differentiate the bone marrow blood endothelial cells from stromal cells and leukocytes. I have many colors in my FACS panel,...
20 January 2023 5,681 1 View
Hello everyone! I am working with DsRed and GFP expressing mice and noticed that there are different cells with different expression levels of DsRed or GFP in a same tissue. For instance, I...
03 January 2022 1,623 3 View
Hello everybody, I'm looking for a tumor cell line (B16 or LLS) expressing OVA protein on the cell surface that I can use with OT I & OT II T cells. Does anyone have them so can share with...
22 May 2020 6,357 0 View
Hello Hello, I am trying to stain DsRed/GFP lymphocytes in recipient tissue. Tissues are frozen spleen embedded in OCT gel. I used a primary Rabbit polyclonal to RFP (Anti-RFP antibody ab62341)...
21 July 2018 8,745 3 View
I am working on trafficking of MDSCs to tumor xenograft (LLC-1 cells at the moment). I am adaptively transfer MDSC from tumor bearing mice to recipient tumor bearing mice and thereafter I track...
07 March 2017 4,630 0 View
I have generated DC from mice bone marrow. I have followed very known protocol, incubating with GM-CSF for 8 days and LPS activation overnight or 48 hours. After LPS activation, I stained my...
16 February 2017 6,441 4 View
I am currently injecting s.c LLC-1 cells or E077 cells into C57BL/6 mice. I am injecting from 100 000 cells to 300 000 cells. The cell viability is around 80-90% before injection. The cells also...
03 February 2017 6,153 12 View
I am working with adaptively transferring leukocytes in tumor-bearing mice. So I am taking the cells from the donor animals, label them with fluorescent dyes and inject them back to the...
02 February 2017 2,841 8 View
I am working with tumor xenografts and preparing single cell suspension! I am currently using 20 mg/ml of collagenase IV, 100 ug of DNase and CaCl2 in plain RPMI medium. I incubate them for 45...
02 February 2017 4,217 2 View
