Sina Tadayon

10 Questions 11 Answers 0 Followers

Questions related from Sina Tadayon

What the hematopoietic stem cell that express Lin- CD117+CD150+Sca1+ and CD48+?

Hello! I have isolated Lin- CD117+CD150+Sca1+. But there are two types of cells here: CD48+ and CD48 -. What is the difference between them? Can I call CD48+ cells more differentiated HSC and...

15 November 2023 5,894 2 View

What is the best medium for culturing human bone marrows?

Hello! I am planning to culture whole human bone marrow for 3 to 4 days and need a culture to maintain all the possible cell types, including mesenchymal stroma cells and their fibroblasts, etc....

10 February 2023 8,396 3 View

Mice bone marrow stromal cell pan marker?

Hello! I am phenotyping the mouse bone marrow, and I only need to differentiate the bone marrow blood endothelial cells from stromal cells and leukocytes. I have many colors in my FACS panel,...

20 January 2023 5,630 1 View

DsRed or GFP expressing mice with differnet DsRed or GFP expression levels?

Hello everyone! I am working with DsRed and GFP expressing mice and noticed that there are different cells with different expression levels of DsRed or GFP in a same tissue. For instance, I...

03 January 2022 1,572 3 View

Looking for a cell line expressing OVA protein???

Hello everybody, I'm looking for a tumor cell line (B16 or LLS) expressing OVA protein on the cell surface that I can use with OT I & OT II T cells. Does anyone have them so can share with...

22 May 2020 6,308 0 View

How to label Ds-Red/GFP cells?

Hello Hello, I am trying to stain DsRed/GFP lymphocytes in recipient tissue. Tissues are frozen spleen embedded in OCT gel. I used a primary Rabbit polyclonal to RFP (Anti-RFP antibody ab62341)...

21 July 2018 8,684 3 View

How the size of tumor xenograft affect the recruitment of MDSCs (specifically M-MDSC and PMN-MDSC) to the tumor?

I am working on trafficking of MDSCs to tumor xenograft (LLC-1 cells at the moment). I am adaptively transfer MDSC from tumor bearing mice to recipient tumor bearing mice and thereafter I track...

07 March 2017 4,590 0 View

What is MHC II high CD 11C low cells?

I have generated DC from mice bone marrow. I have followed very known protocol, incubating with GM-CSF for 8 days and LPS activation overnight or 48 hours. After LPS activation, I stained my...

16 February 2017 6,385 4 View

A feasible way to label the neutrophils in vivo in tumor-bearing mice?

I am working with adaptively transferring leukocytes in tumor-bearing mice. So I am taking the cells from the donor animals, label them with fluorescent dyes and inject them back to the...

02 February 2017 2,785 8 View

What is the best way to dissociate tumors?

I am working with tumor xenografts and preparing single cell suspension! I am currently using 20 mg/ml of collagenase IV, 100 ug of DNase and CaCl2 in plain RPMI medium. I incubate them for 45...

02 February 2017 4,160 2 View