I need to go for Real Time PCR expression studies...

Well, I usually used the RNAplus protocol from MP Biomedical for this. The Qiagen kit is good too... and faster.

you can use Universal RNA Purification Kit from Eurx

kindly see attach

See attach

What are you purifying from? Tissue? Biofluid? What type and quantity?

The Qiagen RNA isolation kit is great for qPCR quality RNA.

I have had good luck with the Qiagen RNeasy for cells and brain tissue.

I still use TriReagent.

Hey! I'm not really experienced in RNA extraction but recently I had to do it in various occasions and Promega's SV Total RNA Isolation System worked really well for me... Fast and easy protocol and a good RNA yield and quality. The price is also fine I guess! PD: I worked with a fungal material.

I've used Qiagen RNeasy mini kit for NGS, and it worked really well.

Qiagen is good, but it works a lot better if you do Trizol extraction and then you combine it with the Qiagen Kit

I attached a protocol that works great for RNA purification especially with large amount of tissue. Good luck!

Trizol.

I would say RNeasy kit from Qiagen. You can also do with Trizol but just give two good washes in 75% ethanol in the last before suspending your RNA in mol bio grade water to ensure complete removal of any residual traces of phenol, salts or any other contaminants. This will take care of your A260/230.

For rna always go for ambion kits

yes, RNAeasy kit from Qiagen :)

Hi Anjan, try Masterpure RNA Isolation Kit from Epicentre, for me is one of the best. Nice RIN numbers. Eventually, you must extent DNAseI step up to 40 min. This tip is always recommended because most of RNA isolations kits do not remove entirely DNA with manufacture's recommendations. Avoid to use column-based kits, they lose a lot of RNA because column saturation and additionally they do not retain pretty well the small RNAs (i.e miRNA or short mRNAs < 200 nt). Good luck!

a combination of Trizol or triPure with Purelink RNA kit from life technologies is the best.

I used Trizol and afterwards the Oligotex mRNA Mini Kit - works fine!

We use RNeasy from Qiagen since a decade and never had problems.

I used trizole and it works very good

You can try Nucleospin RNA from Clontech.

Here is the comparison compared to other kits

http://www.clontech.com/US/Support/Applications/Nucleic_Acid_Purification/Total_RNA_Purification

Trizol! Fast & easy, although there will be some contaminants. I usually extract with trizol then purify the RNA with the Life Technologies PicoPure RNA isolation kit. According to the kit it can be used for extraction too, but it didn't work out as well for me as it did when I extracted with trizol.

Definitely wouldn't recommend trizol for protein isolation though....

I will also recomand for RNA easy kit from qiagen.

Definitely, if you want a fast kit with good yesld and high purity, I would suggest the RNA easy kit from Qiagen. There are different type basing on the amount of starting material you have, and you can perform a DNAse digestion on column if needed (but you will need to buy the DNAse I separately).

For the RNA world, AMBION products are the first-class :)

Trizol is the best choiche for almost every application

I used a kit from Qiagen and it works very good

i used Quagen, Sigma, and Trizol based kit form Invitrogen, and found the result of Trizol is the best. If you are not did before just try with Trizol.

Conventional method is the best one: Trizol treatment followed by chloroform treatment and ethanol precipitation.

Generally will results very good yields

There are several of them but i now rely on RNA extraction kit by Jena Biosciences, it is cheap faster and gives high yield of RNA. I just ordered for another 250 reaction kit and it cost me less than Euro 400 minus shipment.

How you handle the sample prior to extraction can make a huge difference in yields too. In the past, I had always snap frozen the tissue or cells with liquid nitrogen. Recently used RNALater from AMbion/Life tech. This helped with yields and reduced degradation. I was dubious about the product before trying it. But the proof is in the pudding. The stuff is cheap and definitely worth it.