Marcin Wegrecki

3 Questions 23 Answers 0 Followers

Questions related from Marcin Wegrecki

Why is a protein eluting in a huge volume range from size exclusion column?

I have been purifying the propeller domain of a protein by HisTrap column followed by gel filtration using Superdex column. The thing is that the elution profile from the SEC column is really...

05 May 2014 6,737 7 View

Why is the sequence corresponding to only one of the domains when amplifying a gene?

I am trying to amplify a gene from a cDNA library that I made after mRNA extraction from a fungus. The library itself doesn't seem to be the problem since in my lab we've already easily amplified...

02 February 2014 8,141 4 View

How do I get an RNA fragment for the co-crystallization of RNA/protein complex?

Does anyone know what is the best/cheapest way of getting a fragment of RNA, up to 60 nucleotides long? In-vitro transription might be OK but I don't really know if it will give enough RNA for the...

11 November 2013 4,182 3 View