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Questions related from Justine Calise
I labeled an antibody with FITC a year ago, titrated it for flow and it gave good results. I aliquoted it and store it at -20C away from light, as the kit recommends. I also have it wrapped in...
08 August 2015 2,526 8 View
I will be doing experiments involving culturing and stimulating PBMCs and harvesting them 24 hours later. Basically, they will only be in culture for a day. I am interested in a potential...
07 July 2015 2,689 5 View
I am doing a one-day assay with primary monocytes and seeding them at 3*10^5/well, as per a colleague recommendation. However I see others recommend much lower, but they seem to have different...
06 June 2015 5,803 3 View
I have been doing cell culture experiments that involve treating the cells with estradiol (E2). There are many papers published that state these cells are responsive to estrogen. The problem is...
05 May 2015 9,182 6 View
I am working on transfecting Ramos cells and currently using electroporation: the optimized protocol for the Amaxa Nucleofector system. The problem is I am not getting any luciferase readouts. I...
04 April 2015 4,057 4 View
I have 3 large files with case control data for various SNPs on my gene of interest. The files are in the format .bed, .bim, and .fam. Apparently Haploview only accepts .ped and .info files. Do I...
03 March 2015 2,790 9 View
As opposed to using Excel for analysis.
01 January 2015 7,718 1 View
I plotted Ct vs log conc. of my standards and the slope of the curve is -1.79, giving me an efficiency of 262%.
04 April 2014 8,885 18 View
I've seen it done either way.
02 February 2014 2,355 8 View
