Anyebe Onoja

6 Questions 23 Answers 0 Followers

Questions related from Anyebe Onoja

Do you have expertise on use of @ RISK?

Looking for collaboration on risk analysis, if you are skilled in using @RISK and interested, kindly send me a personal mail [email protected]

07 July 2018 5,378 3 View

How sensitive are mice to dengue virus?

I want to isolate/build the titre of dengue virus in mice, The mosquitoes were caught and stored at -56°C for a long time. Please who has experience in the technique of mice inoculation with...

09 September 2014 4,556 8 View

Can anyone help with troubles relating to the loading tray of real time PCR?

After using the qPCR days before for a multiplex reaction, I created another run template for a singleplex reaction and set the thermal profile. As I started the reaction, an error message from...

08 August 2014 3,078 3 View

Why am I unable to get a PCR band size i got earlier?

I used a protocol to amplify a segment of a viral RNA. After making cDNA, I was able to detect the positive control four times with excellent bands, each time i used the same reagents including...

08 August 2014 7,944 8 View

How can I get rid of unspecific binding in conventional PCR?

I obtained very sharp bands in the region I am amplifying, but there are some faint bands below still (I guess primer dimers). I raised and lowered the temperature by one degree, for the +1degree...

08 August 2014 529 7 View

Can someone kindly help me with details, website where I can buy a kit for Yellow Fever Virus IgM and/or IgG ELISA?

05 May 2014 3,153 2 View