Regarding GFP tagging
You may have look this article....Establishment of an efficient genetic transformation system in Scenedesmus obliquus.
Tried pET 3a and it worked.
You can try either with pET30-R5-GFP or pET GFP LIC cloning vector. You can find the database here:
https://www.addgene.org/17838/
https://www.addgene.org/29716/
Best of Luck!!
Protein expression, E. coli
08 September 2014 946 1 View
Regarding viral nucleic acid maintentance
06 July 2014 1,681 6 View
Regarding PCR
05 June 2014 8,708 7 View
Regarding cDNA synthesis.
04 May 2014 5,309 4 View
.
03 April 2014 7,474 9 View
Molecular Taxonomy related
01 February 2014 5,295 3 View
RNA extraction.
01 February 2014 2,530 32 View
11 December 2013 4,018 2 View
PCR related
05 June 2013 5,316 10 View
Relating to gene expression studies.
03 April 2013 3,726 5 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View