Please suggest an efficient protein expression vector for E. coli

08 September 2014 899 1 View

How does viruses keep their nucleic acid materials well intact even when they are not inside any host i.e. outside the host?

Regarding viral nucleic acid maintentance

06 July 2014 1,600 6 View

What should be the quality of water that is considered suitable for PCR reactions? Please suggest!

Regarding PCR

05 June 2014 8,624 7 View

Can anyone suggest a suitable vector with GFP that can be expressed in E. coli?

Regarding GFP tagging

04 May 2014 1,358 3 View

What is the best kit for Real Time PCR expression studies?

.

03 April 2014 7,377 9 View

What is the best kit/protocol for getting highly purified, good quality RNA?

RNA extraction.

01 February 2014 2,444 32 View

Why do we consider 16SrDNA sequence for identification of bacteria at species level and not 23SrDNA or 5SrDNA sequences?

Molecular Taxonomy related

01 February 2014 5,241 3 View

Can anyone suggest or provide literature on 'Genetic basis of Innate Immune system'?

.

11 December 2013 3,975 2 View

Is it possible to amplify a ~10 kb DNA sequence uninturruptedly?

PCR related

05 June 2013 5,258 10 View

Is it possible to synthesize cDNA directly by Colony-PCR with reverse-transcriptase, using appropriate primer for an expressed gene?

Relating to gene expression studies.

03 April 2013 3,668 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View