Daleep Arora

5 Questions 15 Answers 0 Followers

Questions related from Daleep Arora

How important is Chop as a marker for apoptosis or can cell be happy even under high level of chop at mRNA and protein level?

I have a very controversial question. Is it possible that a compound increases chop in a dose dependent manner in parallel decreases all other markers for apoptosis such as Cleaved caspase-3, Parp...

03 March 2015 2,609 6 View

Does anybody know of ATF6 (Activating transcription factor 6) antibodies that have worked in INS-1 cells for western and immunostaining?

I am planning to look at the expression of ATF6 in INS-1 cells under high glucose or ER stress. There are number of antibodies out there but I want to know if anyone in researchgate has personally...

07 July 2014 1,558 2 View

What is the reliable and quick method to isolate RNA from 384 well plate?

We are interested in identifying compounds that can either increase or decrease the expression of our gene of interest. We usually seed cells in 384 well plates and then incubate cells with stress...

03 March 2014 8,325 3 View

What is the best housekeeping gene that can be used to study the effect of low and high glucose on the expression of various genes in INS-1 cells?

I am trying to analyze the expression of some genes under low and high glucose treatment in INS-1 cells. Most of the housekeeping genes such as actin or GAPDH seems to change a bit under these two...

03 March 2014 3,887 15 View

Why do we need to starve cells in low glucose serum media overnight before doing high glucose treatment?

What does low serum do to the cells?

02 February 2013 1,304 3 View