What is the best concentration of MitoTracker dyes that used for mitochondria?

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What are the differences between thershold and Signal to noise in GC or HPLC?

what are the differences between thershold and Signal to noise in GC or HPLC?

26 June 2023 7,400 2 View

Hello how do you find shrine museum s as a sacred places ?

i mean how can we do it

15 April 2019 6,910 2 View

Can I use Amicon centrifugal ultra filter device for coliphage purification?

Are this method is useful for coliphage ? What the precise MOW of these devices 10kda or 100kda

11 August 2018 4,660 6 View

Slip cast of fused silica, too much shrinkage after drying in plaster mold. Any idea about how to control the shrinkage?

mean particle size of FS is about 11 micron. The shrinkage is appeared at the top of the mold due to settling of FS.

23 May 2017 199 2 View

How is it possible to commercially and cost-effectively separate Helium from Argon gas?

there is need to Helium be separated from Argon in some processes .

30 September 2012 7,743 6 View

Any specific peptide based ligand for mitochondrial targeted delivery of nano-drug delivery systems?

Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...

20 June 2024 9,587 1 View

How to enumerate mitochondria? and how to isolate them from a single cell suspension?

I am interested in analyzing the effects of some immunotherapy on the number of mitochondria in cancer cells as well as in immune cells. I would like also to isolate mitochondria to use them for...

16 June 2024 9,632 1 View

Staining of isolated mitochondria tecnique?

Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated...

07 June 2024 5,909 0 View

How to analyze and quantify mitotracker confocal images?

I have been trying to study the mitochondrial morphology using mito tracker dye. But unfortunately I have not been able to find a way to analyze the images. Could anyone give any advice on any...

02 June 2024 6,183 0 View

Histology - mitochondria: Hi RG members. Is anybody aware whether a reliable method for determining mitochondrial density exists for light microscopy?

I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...

02 June 2024 5,373 2 View

How can I detect a small amount of Protein without overexpression?

Hi, are there other methods beside Western Blotting to detect a specific protein? I‘ve isolated mitochondria and want to show that hPar17/Pin4 is present in mitochondria. The Western Blots didn’t...

24 May 2024 5,500 3 View

What fluorescent dye for mitochondria of "dead" multicellular organisms?

Is there a dye and method that could be effective for staining the mitochondria of isolated insect embryos from eggs for confocal microscopy imaging? We are having trouble finding an appropriate...

21 May 2024 4,033 4 View

Comparing ATP Production Rates: Chloroplasts vs. Mitochondria in Photoautotrophic Cells?

In photoautotrophic cells under light conditions, what proportion of the total ATP is generated within the chloroplasts, as a rough estimate or within a lower and upper bound, and what proportion...

14 May 2024 6,492 2 View

Mitochondrial membrane potential using Rhodamine 123 ?

I am determining the mitochondrial membrane potential of mitochondria (isolated from rat brain tissue) using Rhodamine 123 dye. In my experimental procedure, i take the reading of...

08 May 2024 5,081 1 View

Why I am not getting accumulated GFP in vacuole for om45 WT GFP yeast strains?

I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...

04 May 2024 6,463 0 View