What is the best concentration of MitoTracker dyes that used for mitochondria?

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

Which is the best mitochondrial potential dye for primary cells?

Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...

12 January 2021 4,200 4 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View

Is it correct to expect the same quantitative result between SDS-PAGE and BN-PAGE analysis performed on the same sample?

Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...

05 December 2020 7,696 2 View

How to prevent protein aggregation in gel filtration (size exclusion) column ?

I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...

29 November 2020 1,483 5 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

Does anyone know of a more affordable alternative to Mito-Tracker, that is well retained in fixed cells?

I have in the past worked with the MitoTracker dies. They are rather expensive and that is the bit I am hoping to avoid. I would really appreciate it if anybody can suggest a mitochondrial...

05 November 2020 5,701 1 View

Why I am losing the Zona of pellocida while doing mouse preimplementation embryo staining?

I am doing some staining experiments in mice embryos and I noticed that I lose the zona of pellocida during the procedures. I can not define at exactly which step I lose it but mostly either...

15 October 2020 2,906 2 View