Is there a dye and method that could be effective for staining the mitochondria of isolated insect embryos from eggs for confocal microscopy imaging? We are having trouble finding an appropriate mitochondrial staining method because it is not possible to isolate live embryos from the eggs, and it is also not feasible to perform imaging or measurements while the embryos are inside the egg shell.
Wioletta Michalik ,
please contact Prof. Michael Börsch (Uni of Jena Germany) for a valuable help. Give in google : Prof. Dr. Michael Börsch and in Research Gate too.
Good luck and a successful research
JRG alias Jean-Rene Grezes
When staining mitochondria in dead (non-viable) multicellular organisms, traditional dyes like MitoTracker, which depends on membrane potential, are not effective. Here are suitable options for labeling mitochondrial structures in dead cells: MitoTracker Green FM: Stains mitochondria in both live and dead cells, independent of membrane potential. and MitoView Green: Labels mitochondria in both live and fixed cells by binding to mitochondrial proteins.
Kais Khudhair al Hadrawi Thank you! I found these dyes, but I wasn't sure if they were suitable. If I have embryos in eggs, must the dye first be injected into the egg and incubated with living cells?
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