I have been trying to study the mitochondrial morphology using mito tracker dye. But unfortunately I have not been able to find a way to analyze the images. Could anyone give any advice on any plugin or software that you have used successfully?
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,482 5 View
I have been using Gromacs 2021.7. I wanted to simulate two small molecules in water. But, while generating the ions.tpr, I am getting the following error. The topol.top and .itp files for each...
17 July 2024 9,897 4 View
and will i add phytochemical along with FUDR?
28 June 2024 6,254 0 View
I want to calculate cluster sizes for a box of particles using the following method: # Compute cluster IDs for atoms compute myCluster all cluster/atom 0.9 # Define chunks based on cluster...
06 June 2024 5,273 2 View
I have analyzed my responses and valid p value was obtained but didnt show df value
26 May 2024 916 2 View
I have a WT sample from which we have sorted various cell populations via FACS. As a control population, we usually sort a pool of mixed cells from the same sample. Using RT-qPCR, I simply want to...
23 May 2024 6,992 1 View
In VASP orbital projected density of states (DOS) can be plotted without introducing spin orbit coupling (SOC). But how spin orbit entangled Jeff = 1/2 and Jeff =3/2 states can be plotted in DOS...
31 March 2024 5,933 0 View
Geographical area: Southern bank of Yarlung Tsanpo, South-Tibet, Bumdelling-Tawang corridor, Eastern Bhutan. Keywords: Non comsumptive-Tourism, Soft Adventure, IBA, CCA, Host community,...
29 March 2024 3,757 0 View
Will there be a change in bandgap energy of AgNP exposed under blue, red and visible wavelengths of light?
22 March 2024 2,316 2 View
How to download the amino acid sequence of a gene from all Arabidopsis ecotypes/accession from 1001 Genomes or any other database?
11 December 2023 1,032 7 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,289 1 View
Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...
19 July 2024 4,866 1 View
These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..
13 July 2024 9,701 1 View
The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?
07 July 2024 8,118 1 View
500 ng RNA is converted to cDNA to be genetically expressed by qpcr sybergreen dye, 1 ul primer F, 1 ul primer R, 10 ul master mix, 1 ul cdna template, and instead of a complete total volume...
06 July 2024 5,477 4 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
30 June 2024 9,299 0 View
I seek to understand the theoretical basis for selecting the optimal temperature for the thermal regeneration process of exhausted adsorbents, such as activated carbon loaded with dye molecules....
28 June 2024 1,867 3 View
how can I incorporate the dyes to each phase ? do I have to heat them !!
21 June 2024 9,377 1 View
Good evening, I´m establishing a protocol for detecting ROS using DHE staining in Flow Cytometry using TK6 cells. They look visibly pink after staining them with 10 micro Molar DHE. Is that...
19 June 2024 7,339 0 View
methylene blue stock solution
19 June 2024 10,030 3 View