Are this method is useful for coliphage ? What the precise MOW of these devices 10kda or 100kda
I have not experience with viruses. Amicon basic method is same Dialysis, so if dialysis can be done amicon can be done too.
I used dialysis for purification but I am not sure about the Amicon filter devices.
These centrifugal ultrafiltration devices can be obtained with various molecular weight cutoffs, all of which are on a molecular scale (10,000 to 100,000 Da). Virus will not pass through.
I read paper indicate that these devices can be used instead CsCl purification and high centrifuge
(Most) phages will not pass through these filters. If you apply a T7 or T4 lysate, for example, almost all phage particles will be concentrated above the MWCO column. If your goal is to concentrate viral particles, this should work, but since it's not impossible for a handful of phages to pass through, your filtrates may or may not be completely phage-free.
We usually use 50K or 100k MWCO to trap the coliphages, followed by CsCl gradient for TEM image because the filter will also capture any large debris from the host lysis and cause noisy background, thanks.
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